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Backbone and insert fragment were ligated and transformed by heat shock into NEB 5 competent cells following the provided standard procedure of the vendor

Vitamin C is also a cofactor in numerous enzymatic reactions involved in the making of collagen, L-carnitine, and several neurotransmitters, and in the regulation of gene expression

On the other hand, the adrenal cortex synthesizes and releases cortisol, which increases gluconeogenesis and insulin resistance to ensure energy production and also plays an essential role in modulating inflammation during the acute phase [104,110]

IECs are a core component of the intestinal epithelial barrier