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3) For large volume of supernatant, concentrate the proteins by ammonium sulphate precipitation, dialyze the dissolved protein solution against 1 PBS, and then apply the solution onto the GSH column

NAC numerically reduced diarrhea incidence ( 46.2%) and the concentrations of hydrogen peroxide and malondialdehyde, but increased claudin-1 and intestinal fatty-acid binding protein (iFABP) protein abundances and activities of catalase and glutathione peroxidase in the jejunum of -CG-challenged piglets

MOTS-c translocates to the nucleus under cellular stress conditions and directly regulates nuclear gene expression relevant to oxidative phosphorylation and antioxidant defense, a mitonuclear communication mechanism unique among cataloged research peptides

Sailer, N