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10.1016/j.metabol.2008.12.003 63

For endogenous immunoprecipitation, the cells were lysed, the lysates were incubated with anti-WDR24 antibody (rabbit IgG used as control) and protein A/G magnetic beads (MCE) overnight at 4 C, washed five times with 0.1% Tween-20 and boiled with SDS loading buffer for 10 min 68

Three PPAR isoforms have been identified so far, commonly referred to as PPAR, PPAR/ , and PPAR, which show different tissue distributions and ligand specificities (70, 71)

Protective roles of O-GlcNAcylation have been reported in cardiac ischemia/reperfusion [218, 219], and the use of glucosamine to bypass GFAT for UDP-GlcNAc production appears to serve anti-inflammatory roles [220, 221]