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For combined confocal/STED imaging of synaptic vesicles and alpha-synuclein, vesicles were stained with a primary antibody for synaptotagmin-1 (105103, 1:500 dilution, SYnaptic SYstems) and a secondary anti-rabbit antibody conjugated with Alexa-488 (18772, 1:100 dilution, Sigma-Aldrich)

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