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1 Introduction Mastitis, an inflammation of the mammary glands, is characterized by increased inflammation, reactive oxygen species (ROS), and reduced immune effectiveness in the mammary gland tissues ( The multifaceted nature of mastitis as a disease is widely recognized in the scientific community ( Staphylococcus aureus , often presents more subtly, with no obvious symptoms except for elevated milk somatic cell counts and a decrease in milk yield ( Escherichia coli, Streptococcus uberis, S