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Shift Away from Dopaminergic Drugs: The AASM advises against the routine use of levodopa, pramipexole, and similar medications, due to evidence linking these drugs to the worsening of RLS symptoms over time

Concentrated fractions were then incubated with 20mM GSSG (Thermo J63715.06) and PMab1 Fv-clasp at a 1:1.5 molar ratio for 30 min at room temperature

This fragmentation slows hypothesis generation, delays mechanistic validation, and weakens the iterative bench-to-bedside process needed to translate molecular findings into meaningful clinical outcomes

The SYBR Green Pro Taq HS premix qPCR assay kit from Accurate Biotechnology Co., Ltd was used for reverse transcription of total RNA