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PLoS ONE 16 , e0260023 (2021)

Iron as a Therapeutic Target for Parkinsons Disease

The proteins were then separated by 12% (w/v) SDS-PAGE and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, USA) using wet transfer at 85 V for 1 h with a BioRad electroblotting apparatus