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Nature (2015) 527(7577):18691

Tyrosylprotein sulfotransferase-1 and tyrosine sulfation of chemokine receptor 4 are induced by Epstein-Barr virus encoded latent membrane protein 1 and associated with the metastatic potential of human nasopharyngeal carcinoma

The membrane was incubated with 5% (w/v) non-fat dried milk as a blocking solution and then probed with the indicated primary antibodies (Merck, USA), including anti-iNOS (1:2000) and anti--actin (1:2000)