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USA 116 , 2022620231 (2019)

[DOI] [PMC free article] [PubMed] [Google Scholar] [74].Modrick ML, Didion SP, Lynch CM, Dayal S, Lentz SR, Faraci FM, Role of hydrogen peroxide and the impact of glutathione peroxidase-1 in regulation of cerebral vascular tone

Briefly, the assay involves the enzymatic conversion of glucose to glucose-6-phosphate coupled with glucose-6-phosphate dehydrogenase, and the NADH thus formed reduces a colorless probe to a colorimetric product, which is then read at 450 nm