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Mn 2+ directly activates cGAS and structural analysis suggests Mn 2+ induces a noncanonical catalytic synthesis of 2'3-cGAMP

Thus, independent of whether the experiments were started with reduced or oxidized proteins, most of strep-MBP-ROXY9 was in the reduced state in the presence of GSH with spurious amounts of GSSG, while strep-MBP-GRXC2 became oxidized, with about half of the proteins in the sample being glutathionylated and half containing the intramolecular disulfide

This work identifies the additional requirement for the microprotein RMSC and its replication-coupled PARylation in human cells