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After washing, the slices were fixed with 4% PFA in PBS for 1 h at room temperature, and the fixation reaction was quenched with 100 mM NH 4 Cl in PBS for 30 min

It is known that ROS could induce both necrosis and apoptosis via activation of Caspase-3 protease, which is cleaved to a p12 subunit and a p17 subunit 38