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Estimation of liver function parameters Aspartate aminotransferase (AST), alanine aminotransferase (ALT) were assayed in serum samples obtained from all groups of mice by a colorimetric method of Reitman and Frankel ( Estimation of iron parameters Serum iron (SI) concentration and total iron-binding capacity (TIBC) were estimated by colorimetric analysis according to the manufacturers protocols for commercial kits (Biodiagnostic, Egypt)

d , Immunoblot analysis of indicated proteins from KPK cells expressing sgRNA targeting intergenic region or sgRNA targeting Slc33a1