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There were, however, slight differences such as one which made efforts to eliminate some other factors that affect GM (Lee et al., Reference Lee, Yun, Kim, Lee, Chang, Ryu, Shin, Kim, Kim and Lee2018), exploration of electronic cigarette (EC) vapour and tobacco smoke exposure (Stewart et al., Reference Stewart, Auchtung, Ajami, Velasquez, Smith, De La Garza, Salas and Petrosino2018), evaluation of the combined effects of cigarette smoking and alcohol consumption (Lin et al., Reference Lin, Zhang, Chen, Qi, He, Hu, Zhang, Fan, Yang, Wang and Si2020), and use of whole-genome sequencing (WGS) to explore the effects of smoking on the GM at the species level (Yan et al., Reference Yan, Ma, Jiao, Wang, Li and Ding2021)

Nicotine is rapidly absorbed but metabolized in the liver into cotinine, a primary by-product

Despite the ongoing body of knowledge that builds the case for everyone to stop smoking, the Centers for Disease Control and Prevention estimates that some 30.8 million adults in the United States smoke (as of 2020)

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