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A protocol for a randomised double-blind placebo-controlled feasibility study to determine whether the daily consumption of flavonoid-rich pure cocoa has the potential to reduce fatigue in people with relapsing and remitting multiple sclerosis (RRMS) Pilot Feasibility Stud

Briefly, hiPSC colonies were detached with collagenase type IV, washed with DMEM:F12, and transferred to an uncoated polystyrene petri dish (VWR) in neural induction medium containing DMEM:F12 supplemented with 20% KnockOut Serum Replacement, 1X MEM-NEAAs, 2mM GlutaMax, penicillin (100 units/mL)/streptomycin (100 g/mL), 0.1mM 2-mercaptoethanol, 2 mM dorsomorphin and 2 mM A83-01

Reconstitution Reference For a 100 mg vial, adding 2 mL of bacteriostatic water yields a concentration of 50,000 mcg/mL

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