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Key considerations for IM injections include: Using a needle of the right length to ensure the medication is delivered into the muscle Rotating injection sites to avoid muscle irritation Administering the injection at a 90-degree angle to the skin surface Subcutaneous Administration Benefits Subcutaneous injections put Vitamin B12 into the fatty tissue just under the skin

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Flow cytometry For viability staining, CD4 + T cells were washed with PBS then stained with Zombie viability dyes (BioLegend) at room temperature for 5 min, and then washed with FACS buffer

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