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In Yarrowia lipolytica this challenge of complexity was tackled by the establishment of an alternative plant-derived astaxanthin biosynthesis from Adonis aestivalis 20 resulting in astaxanthin as the exclusive hydroxylated carotenoid due to the sequential activity of carotenoid 4-hydroxy--ring 4-dehydrogenase (HBFD) and carotenoid -ring 4-dehydrogenase (CBFD)

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Future directions may involve the development of engineered probiotics with improved tissue targeting, personalized FMT protocols based on metagenomic and metabolomic profiling, and applying machine learning to predict individual responses