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PCR genotyping of the sorted cells with the specific primers ( Table 1 ) demonstrated that the F4/80-positive cells, but not the negative ones, had specific Cftr -exon-10 deletion ( Supplementary Figure 1B ), indicating that the Mac-CF mice had the designed Cftr -exon-10 deletion in macrophages alone

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mTORC1 activation on membranes We used large unilamellar vesicles (LUVs), with a lipid composition of 72.8% DOPC, 7% POPS, 10% cholesterol, 5% DGS-nitrilotriacetic acid (NTA), 5% PE MCC and 0.2% DiR, as the membrane platform to investigate the role of RAGRagulator and RHEB in mTORC1 activation

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