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In the third route, either the 5- or 3-end of the cDNA template encodes a ligase ribozyme sequence such that during in vitro transcription, the resultant nucleic acid molecule can contain an active ribozyme sequence capable of ligating the 5-end of a nucleic acid molecule to the 3-end of a nucleic acid molecule

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This matters if you frequently forget to refrigerate your pens or if you travel often