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Published human data includes a small 2017 study in the International Journal of Molecular Sciences reporting improved skin firmness after topical GHK-Cu application over 12 weeks

There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes

by Chris Kresser [ 2