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This study determined whether co-injections of L-NAC (500 mol/kg, IV) or its highly cell-penetrant analogue, L-NAC methyl ester (L-NACme, 500 mol/kg, IV), prevent acquisition of acute physical dependence induced by twice-daily injections of fentanyl (125 g/kg, IV), and overcome acquired dependence to these injections in freely-moving male Sprague Dawley rats

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Furthermore, it inhibits inflammatory cascades by suppressing the COX enzyme and prostaglandin E-2 (PGE2), also influencing various molecular signaling pathways related to inflammation, apoptosis, and oxidative stress, like PPAR, PI3K, TLR-4, Akt, mTOR, ERK5, AP-1, TGF-, PAK1, Wnt, -catenin, Shh, Rac1, p38MAPK, EBP, NLRP3 inflammasome, Nrf2, Notch-1, AMPK, STAT3, and MyD-88, crucial in IBD development [504]