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However, we found that it is virtually fully oxidized, when expressed in the periplasm, suggesting that its engineered cysteine residues are completely oxidized by the oxidative folding machinery

Molecules 20, 1888618906

In a circadian misalignment model (mice kept in the dark for 14 days) in which lower SIRT1 activity was observed, SIRT1 overexpression led to improved insulin sensitivity [31]