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To improve the expression, solubilization, and purification of recombinant proteins, it is therefore advised to alter the culture conditions by switching the medium or the temperature, co-expressing chaperones, adding large hydrophilic fusion tags as well as adding sucrose, raffinose, glycine, betaine and sorbitol during growth for enhancing the recombinant protein expression, solubilization and purification [5, 6]

PMID: 95 LanLWeiWZhengYNiuLChenXHuangDet al

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It is also needed for the liver to convert T4 into the active T3 form of thyroid hormone, which all the cells in the body need to function properly