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Given the potential impact of these factors on the quantification of GSH, here we examined if the type of anticoagulant used during sample collection (heparin or EDTA), deproteinization of plasma prior to storage (yes or no), storage temperature (80 o C or 4 o C), and processing time (duration of time between collection of whole blood and deproteinization of plasma) impact levels of free GSH and GSSG, in plasma

Vegetarians and vegans are also at risk because B12 is mainly in animal products

The samples were diluted in RIPA, mixed with Laemmli buffer (Bio-Rad, Hercules, CA), and then denatured at 95C for 5 min

Our functional enrichment and gene set enrichment analyses revealed differences in metabolic pathways, particularly fatty acid metabolism, between hepsin / and wild-type mice following APAP exposure