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Patients in their 40s and 50s who have never addressed the GHK-Cu decline often present with compounded effects: skin that has thinned significantly, hair that has changed in texture and density over years, and a recovery timeline after procedures that is noticeably longer than it was in their 20s

2.9 VE-cadherin imaging After 72 h of incubation, treated and untreated sample inserts with HUVECs were washed with PBS and fixed in 4% paraformaldehyde (Sigma Aldrich, United States) for 20 min at room temperature