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The resulting disulfide in the Grx active site, however, is not reduced by a dedicated reductase, but by two molecules of GSH that reduce the Grx via a Grx active site and GSH (Grx-S-SG) mixed disulfide intermediate (Figure 1)

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While cytosolic and blood GSH and Cys biochemical species all experience depletion, the model predicts that a single exposure to doxorubicin only results in a minimal perturbation of the antioxidant defence system