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Materials and Methods Cell lines and culture conditions Certified glioma cell lines U87MG and U343MG (p53 wild-type), U251MG (273Arg-His homozygous p53 mutation) and U138MG (273Arg-His heterozygous p53 mutation) were a kind gift from Bernd Kaina (Mainz, Germany), and were routinely grown in DMEM (Invitrogen, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal calf serum (Cultilab, Campinas, Brazil) and 1% antibioticantimycotic at 37 C in a humidified, 5% CO 2 atmosphere

Fer-1 did, however, prevent erastin-induced accumulation of cytosolic and lipid ROS ( FIG

Therefore, businesses must remain vigilant to ensure compliance with various state laws

These chemicals not only contribute to environmental pollution but also pose risks like toxicity and carcinogenicity (Hua et al., 2018)