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Although the primary function of mitochondria is to generate ATP as an energy molecule required for countless cell functions, a small fraction of electrons from the ETC are transferred directly to O 2 , resulting in the generation of the superoxide anion, which can give rise to other ROS as well as reactive nitrogen species (RNS)

Cells were extracted with 200 L of ice-cold 80% methanol, centrifuged at 15,000 g for 20 min at 4C and lyophilized

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