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TSA was carried out using a BioRad CFX Connect qPCR instrument with a melt curve setting of 25 95 C, in increments of 0.5 C/10 seconds

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The deregulation of NO synthesis (both up- and down-regulation, depending on the arsenic concentration) is interesting because this is a signal messenger that promotes vascular relaxation, but during pathological processes, it generates nitrogen reactive species and oxidative stress, damaging the endothelium (138, 139)

The fairest one-line summary is that DSIP appears to have real biological activity but a weak and inconsistent clinical efficacy signal