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Research has revealed that ROS which linked to the development of male infertility, plays a critical role in the disruption of the spermatogenesis process i.e., diminished ability of the genital systems and sperms antioxidant system (105, 106)

Quantification of the percentage of Safranin-O positive areas was performed using ImageJ (version 1.53t, National Institutes of Health, Bethesda, MD, USA)

Firstly, long-term exposure to air pollutants (PM2.5, NO, ozone) increases intestinal permeability, systemic inflammation, and dysbiosis [221], The UK Biobank cohort has confirmed that PM2.5 and NO significantly increase the risk of UC [222], Other studies have also revealed a correlation between PM2.5 exposure and increased rates of intestinal resection and mortality [223]