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Subsequently, samples were concentrated to dryness in vacuum before dissolving in 150 L of 80% methanol (precooled at -20C), then centrifuged again at 13 500 g and 4C for 10 min to obtain the supernatant for high-performance liquid chromatography Ultimate 3000 (Thermo Fisher Scientific, USA)-tandem mass spectrometry Q Exactive Focus (Thermo Fisher Scientific, USA) (LC-MS/MS) analysis (95, 96)

Even when chest pain seems noncardiac, certain symptoms always require immediate evaluation

Tendons can also be tricky things and sometimes only tell you hours after an activity or the next day that they didnt like it

J Vet Med A Physiol Pathol Clin Med 54, 599-606