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For the dot blot assay, positively charged nylon membrane was first bathed in DEPC-treated water for 10 min followed by DEPC-treated 6 SSC buffer for at least 15 min

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b , Table of apparent MichaelisMenten parameters

The ratio of Firmicutes to Bacteroidetes is a commonly-studied change with an increase often linked to HFD consumption/obesity in mice 43,44 and humans 45,46 (for review, see John and Mullin, 2016 47 )