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Murry and colleagues first introduced the concept, documenting a protective effect of brief ischemic episodes applied before subsequent sustained heart ischemia in dogs ( Oxidative stress inhibition One of the major issues related to perinatal HI brain injury is oxidative stress resulting from impaired mitochondrial functioning that leads to the overproduction of reactive oxygen species (ROS), such as singlet oxygen, hydroxyl radical, superoxide anion, hydrogen peroxide, and nitric oxide

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Especially, glycolysis, HIF-1 signaling, NADH metabolism, and oxidative stress were affected