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Primary antibodies were anti-SOX10 (guinea pig, Hangzhou Normal University,1:200), monoclonal CC1 (mouse, Calbiochem OP80, 1:200)

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Protocol 4: nasal spray approach Goal: Needle-free administration with acceptable bioavailability trade-off Duration: 20 days active, then 4-6 months off Schedule: Days 1-20: 10-15 mg epitalon intranasally, divided into 2-3 daily doses Days 21-180: Off cycle Repeat 1-2 times per year Formulation: Reconstitute in saline with 3 percent DMSO for improved nasal absorption