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Samples for qRT-PCR were assembled using the Takyon No ROX SYBR MasterMix blue dTTP (Eurogentec, Seraing, Belgium) in a total reaction volume of 5 L (2.5 L Takyon MasterMix, 0.125 L forward/reverse primer (10 M), 2 L cDNA (1:10), 0.5 L nuclease-free water)

Similarly, CDs/g-C 3 N 4 /ZnO nanocomposites exhibit highly enhanced photocatalytic activities for the degradation of tetracycline (Guo et al., 2017)

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In other words, increased intracellular iron concentrations stimulate the expression of APP mRNA and thus may enhance A formation (Rogers and Lahiri 2004)