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The DNA encoding the monoclonal antibodies of the invention can be readily isolated and sequenced using conventional procedures (e.g., by using oligonucleotide probes that are capable of binding specifically to genes encoding the heavy and light chains of murine antibodies, or such chains from human, humanized, or other sources)

Clarified supernatants were filtered using 0.2-m filters, transferred to new 50-ml Falcon tubes containing Ni-NTA resin equilibrated with lysis buffer (20 mM Tris-HCl (pH 9), 10 mM imidazole, 200 mM NaCl and 5% glycerol) and agitated for 1 h to allow protein binding

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In vivo research by Matouk et al