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Careful contouring here helps define that crucial transition zone for a more sculpted look

Endogenous kynurenines as targets for drug discovery and development

For the E-64d cocrystal structure, where density in the 2 F o F c maps failed to cover all of the ligands aliphatic tail moieties, the pose of the ligand that was best accommodated by the density and gave the lowest R free in refinement was selected and a feature-enhanced 2 F o F c map was calculated in PHENIX from the original reflections and the optimized model to better justify ligand placement 85

The MAPK family comprises three major subfamilies: ERK (a pro-survival kinase activated by mitogenic signals), and the stress-responsive MAPKs, JNK and p38 (Sugiura et al., 2021)