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Twenty-four of the 46 depressed patients (52%) had raised total plasma homocysteine (Figure 3)

38 ElnaggarA.TsombouF

Membranes were blocked in 5% non-fat dry milk with TBST for 30 min at room temperature and incubated overnight at 4 C with primary antibodies (Supplementary Table 2) diluted in either 5% milk-TBST or 5% bovine serum albumin (BSA)-TBST for phospho-specific antibodies