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The standard body surface area scaling method, endorsed by the FDA for first-in-human dose estimates, typically divides the rodent dose by a factor of approximately 6.2 when converting from rat to human

Roswell Park has a video on how to give a subcutaneous injection

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Once anesthesia was induced using 5% isoflurane, cardiac blood collection was performed, followed by the rapid excision of prostate tissue, which was subsequently washed with pre-chilled 1.15% KCl solution