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Enteric pathogens often gain access to the body by altering the structure and function of tight junctions to increase permeability of the barrier via the secretion of proteases, which can cleave tight junction proteins or by altering the cytoskeleton [116]

Current antifungal drugs Currently, the first-line clinical antifungal drugs include polyenes (e.g., amphotericin B), azoles (e.g., fluconazole) and echinocandins (e.g., caspofungin) (Wiederhold, 2017

The oocytes were subsequently incubated in a permeabilizing solution (0.5% Triton-X-100 in PBS-PVA) for 30 min at room temperature, washed twice in PBS-PVA, and then blocked with 2% FCS in PBS for 45 min

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