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94 Previous studies have shown that the formation of cell-filled ALG microspheres from an aqueous solution of ALG gel precursor and CaCl 2 using the LIFT technique and the formation of a thick gel film during droplet descent significantly increased fibroblast activity, but at the same time, this impeded the diffusion of nutrients and oxygen through the medium, resulting in a decrease in cell viability, and that cell viability decreased with increasing laser fluence or ALG concentration

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