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Next, we wanted to understand how these bHLH TFs were modulated by the GSH-GSNO module

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For AAV-mediated knockdown studies, 8-week-old male C57BL/6 mice received retro-orbital injections of adeno-associated virus serotype 8 (AAV8) carrying either a scramble control or Gpr146-targeting shRNA under the U6 promoter