glutathione stransferase tag s-transferase sequence The role of glutathione-S-transferase in anti-cancer drug resistance Removal of GST Tag by – glutathione-s-transferase-tag purification Affinity tags |

To prepare non-reduced samples, -mercaptoethanol was omitted from loading buffer and samples were loaded without boiling

The importance of targeting glutathione levels is relevant because significant differences in glutathione and its related metabolites have been found in plasma (16, 17), white blood cells (58, 59), and post-mortem brains (11, 21) of participants with ASD

10.1161/01.CIR.0000052627.99976.18 222

They primarily arise from various enzymatic and chemical reactions, including those involving cyclooxygenase, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX), xanthine oxidase, lipogenesis, and the iron-catalyzed Fenton reaction (Tejero et al., 2019), These processes generate hydroxyl radicals (-OH), superoxide radicals (O 2 --), and hydrogen peroxide (H 2 O 2 )