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The reaction was incubated at 40 C for 10 min and terminated by boiling at 100 C for 10 min

Discussion Decellularizing kidneys is complex due to the vast number of cells and intricate structure

In contrast to prior studies 9,12,14,15 , which primarily relied on baseline or intention-to-treatstyle exposure definitions, AHM exposure in the present study was measured throughout the follow-up, and participants with high exposure ( 80% PDC) of the medication class were grouped under the class and compared, an approach not utilised in previous studies 9,12,13,15,31

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