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In order to optimize the liquid chromatography processes , the OMNICOLL fraction collector and reliable LAMBDA peristaltic liquid pumps are successfully used in laboratory practice: Example setup of automatic fraction collection For example, a complete arrangement of column chromatography with automatic collection of eluate using OMNICOLL fraction collector includes: Two similar containers with elution buffer A & B connected by a siphon (or tube at the bottom with a valve to adjust the flow) Elution buffer A is consistently drawn into the beaker with elution buffer B which is constantly mixed by magnetic stirrer , thereby altering its buffer concentration Programmable LAMBDA peristaltic pump to pump the gradient mixture into the column Eluate from the column is being passed through the detector (to measure pH, Absorbance 280 nm, conductance) From the detector, the protein fractionation is collected by the OMNICOLL fraction collector into the desired containers (e.g.: tubes, vials, etc.) The proteins are eluted by changing the composition of the buffer (ionic strength, pH, conductivity, concentration of salts, etc.)

ACS Nano 4 , 36893696, doi:10.1021/nl052396o (2010)

[DOI] [PubMed] [Google Scholar] 141.Yamada M., Elias M.D., Matsushita K., Migita C.T., Adachi O