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The QuickChange Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., CA, United States) was used to develop point mutations

The fixative was discarded followed by a new fixative for electron microscopy, and the cells were observed under TEM (Hitachi)

Briefly, TBS-soluble fractions were chromatographically separated using a Bio SEC-3 column (4.6 300 mm, 3 m, 5190 2508, Agilent), operated at 25 C

The nutritional approach consists of consuming food rich in Glutathione precursors such as cysteine, selenium, and sulfurgarlic, onion, peppers, broccoli, and cabbage, for example, are foods rich in cysteineor also food supplements of Glutathione