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2 Materials and methods 2.1 Animal management, diets, and experimental design A total of 128 growing-finishing pigs (Duroc Landrace Yorkshire) with an initial average body weight of 33.88 0.62 kg were assigned to four treatments in a randomized complete block design based on body weight

In the sham group, animals were subjected to the same procedure, except for the insertion of the nylon monofilament

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In vivo, zinc deficiency does not independently induce ER stress, yet under pharmacologically induced ER stress conditions, zinc-deficient mice exhibit heightened activation of the pro-apoptotic p-eIF2ATF4CHOP axis, accompanied by apoptosis, steatosis and liver injury