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Description

All cells were cultured in RPMI-1640 medium (Biowest, Nuaill, France) supplemented with 10% fetal bovine serum and 1% penicillinstreptomycin at 37 C in a humidified incubator at 5% CO 2 and were passaged every 23 days

HBSS Hanks Balanced Salt Solution H 2 DCFDA 25 M C11-BODIPY(581/591) 2 M MitoSOX 5 M Cell viability was typically assessed in 384-well format by Alamar Blue (Invitrogen) fluorescence (ex/em 530/590) measured on a Victor3 platereader (Perkin Elmer, Waltham, Mass.)