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Subsequently, a 20-g protein aliquot of each sample was mixed with an equal volume of 2 Laemmli sample buffer (Cat # 1,610,737, Bio Rad, California, USA), separated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis, and then conveyed onto a polyvinylidene difluoride membrane

However, its low bioavailability and rapid metabolism limit its clinical translation (46)

However, most studies indicate that consumption of EDs leads to episodes of increased blood pressure and accelerated and increased cardiac contractility