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Injectable formulations typically contain pharmaceutical-grade excipients such as sterile water for injection, buffering agents, and stabilizers designed to preserve molecular integrity

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The nested PCR, which generated DNA fragments of 165-353 bp, was found to be able to detect 1-2 copies of the target DNA, and evidenced no cross-reactivity with the genomic DNA of related microorganisms or of human cell lines, thereby confirming the sensitivity and specificity of the primers used

28 VNS can also enhance motor and cognitive recovery, reduce blood-brain barrier breakdown, and confer neuroprotective effects