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Recently, a sub-chronic whole-body exposure of e-liquid (2 h/day, 5 days/week, 30 days) containing PG alone or PG with nicotine (25 mg/mL) to wild type (WT) animals or knockout (KO) mice in 7 nicotinic acetylcholine receptor (nAChR7-KO) revealed a partly nAChR7-dependent lung inflammation [42]